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goat anti mouse flt4  (R&D Systems)


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    R&D Systems goat anti mouse flt4
    Goat Anti Mouse Flt4, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 218 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vegfr3/Mouse+VEGFR3%2FFlt-4+Antibody/bio_rxiv__64898__2026__03__17__712362-30-81-84
    Average 95 stars, based on 218 article reviews
    goat anti mouse flt4 - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Data-independent acquisition:

    Article Title: Embryological cellular origins and hypoxia-mediated mechanisms in PIK3CA -driven refractory vascular malformations
    Article Snippet: .. The sections were immunostained using primary antibodies against PECAM (DIA-310, Dianova, 1:100, RRID:AB_2631039; M0823, DAKO, 1:100, RRID:AB_2114471), Prox1 (11-002, AngioBio, 1:100, RRID:AB_10013720; AF2727, R&D Systems, 1:100, RRID:AB_2170716), VEGFR3 (AF743, R&D Systems, 1:100, RRID:AB_355563), GFP (ab290, Abcam, 1:250, RRID:AB_303395), phospho-S6 (#2215, CST, 1:100, RRID:AB_331682), Ki67 (ab15580, Abcam, 1:250, RRID:AB_443209), D2-40 (anti-PDPN) (413151, Nichirei Biosciences, ready to use), HIF-1α (ab114977, Abcam, 1:100, RRID:AB_10900336), VEGF-A (ab52917, Abcam, 1:100, RRID:AB_883427; ab51745, Abcam, 1:100, RRID:AB_2256948), and VEGFR2 (#2479, CST, 1:100, RRID:AB_2212507). .. For the enzyme-antibody method, the secondary antibody from the Histofine Simple Stain System (Nichirei Biosciences) was incubated with the slides for 1 h. Peroxidase activity was visualized using DAB-H 2 O 2 .

    Article Title: Embryological cellular origins and hypoxia-mediated mechanisms in PIK3CA -Driven refractory vascular malformations
    Article Snippet: .. The sections were immunostained using primary antibodies against PECAM (DIA-310, Dianova, 1:100, RRID:AB_2631039; M0823, DAKO, 1:100, RRID:AB_2114471), Prox1 (11-002, AngioBio, 1:100, RRID:AB_10013720; AF2727, R&D Systems, 1:100, RRID:AB_2170716), VEGFR3 (AF743, R&D Systems, 1:100, RRID:AB_355563), GFP (ab290, Abcam, 1:250, RRID:AB_303395), phospho-S6 (#2215, CST, 1:100, RRID:AB_331682), Ki67 (ab15580, Abcam, 1:250, RRID:AB_443209), D2-40 (anti-PDPN) (413151, Nichirei Biosciences, ready to use), HIF-1α (ab114977, Abcam, 1:100, RRID:AB_10900336), and VEGF-A (ab52917, Abcam, 1:100, RRID:AB_883427; ab51745, Abcam, 1:100, RRID:AB_2256948). .. For the enzyme-antibody method, the secondary antibody from the Histofine Simple Stain System (Nichirei Biosciences) was incubated with the slides for 1 hour.

    Article Title: Embryological cellular origins and hypoxia-mediated mechanisms in PIK3CA-driven refractory vascular malformations.
    Article Snippet: .. The sections were immunostained using primary antibodies against PECAM (DIA-310, Dianova, 1:100, RRID:AB_2631039; M0823, DAKO, 1:100, RRID:AB_2114471), Prox1 (11-002, AngioBio, 1:100, RRID:AB_10013720; AF2727, R&D Systems, 1:100, RRID:AB_2170716), VEGFR3 (AF743, R&D Systems, 1:100, RRID:AB_355563), GFP (ab290, Abcam, 1:250, RRID:AB_303395), phospho-S6 (#2215, CST, 1:100, RRID:AB_331682), Ki67 (ab15580, Abcam, 1:250, RRID:AB_443209), D2-40 (anti-PDPN) (413151, Nichirei Biosciences, ready to use), HIF-1α (ab114977, Abcam, 1:100, RRID:AB_10900336), VEGF-A (ab52917, Abcam, 1:100, RRID:AB_883427; ab51745, Abcam, 1:100, RRID:AB_2256948), and VEGFR2 (#2479, CST, 1:100, RRID:AB_2212507). .. For the enzyme-antibody method, the secondary antibody from the Histofine Simple Stain System (Nichirei Biosciences) was incubated with the slides for 1 h. Peroxidase activity was visualized using DAB-H2O2.

    Staining:

    Article Title: The thrombin receptor PAR1 orchestrates changes in lymphatic endothelial cell junction morphology to augment lymphatic drainage during lung injury
    Article Snippet: .. Slides from paraffin-embedded sections were stained with hematoxylin and eosin or immunostained as described previously , with the following antibodies: VEGFR3 (1:50 dilution, catalogue number AF743, R&D Systems); CCL21 (1:50 dilution, catalogue number AF457, R&D Systems); or RFP (1:100 dilution, catalogue number 600401379S, Rockland). .. Immunofluorescence was performed using a Nikon Eclipse microscope (×20 objective) with the NIS-Elements software.

    Incubation:

    Article Title: VEGF-C-mediated Cardiac Lymphangiogenesis Promotes Inflammation Resolution in Autoimmune Acute Myocarditis in Mice
    Article Snippet: .. Sections were then incubated with the following primary antibodies: D2-40 (anti-Podoplanin) (413151, Nichirei Biosciences, at its original concentration (ready to use product)), VEGFR3 (R&D Systems, AF743, 1:150, RRID: AB_355563), CD68 (Cell Signaling Technology, 97778, 1:150, RRID: AB_2928056), CD3 (Abcam, ab5690, 1:150, RRID: AB_305055), CD4 (Abcam, ab183685, 1:150, RRID: AB_2686917), CD8 (Abcam, ab209775, 1:150, RRID: AB_2860566), Foxp3 (Abcam, ab215206, 1:100, RRID: AB_2860568), CD20 (Abcam, ab64088, 1:300, RRID: AB_1139386), CD11c (Cell Signaling Technology, 97585, 1:100, RRID: AB_2800282), iNOS (Cell Signaling Technology, 68186, 1:500, RRID: AB_3662912), Arginase1 (Cell Signaling Technology, 93668, 1:100, RRID: AB_2800207), CCR2 (Abcam, ab273050, 1:150, RRID:A AB_2893307), Galectin-3 (Cell Signaling Technology, 89572, 1:100, RRID: AB_2800111), SPP1 (Cell Signaling Technology, 88742, 1:100, RRID: AB_3107209), MRC1 (Abcam, ab64693, 1:100, RRID:AB_1523912), and Ki67 (Abcam, ab15580, 1:250, RRID: AB_443209). ..

    Article Title: Supporting Information for Fibrillin-1 regulates endothelial sprouting during angiogenesis
    Article Snippet: .. Lysis was performed directly in Laemmli sample buffer and samples were run on a 10% SDS-PAGE gel at 120 V for 90 min. Proteins were transferred onto Immobilon polyvinylidene difluoride membranes (Merck Millipore) at 120 V for 1 h. Membranes were saturated with 5% bovine serum albumin in Tris-buffered saline containing 0.1 % Tween 20 for 1h and incubated overnight at 4°C with primary antibodies against fibronectin (Abcam, #ab6328), Dll4 (Cell Signaling Technologies (CST) #2589), Jagged-1 (CST #70109), Smad1 (CST #9743), Smad6 (Novus, #NB100-56440), MAGP1 (Santa Cruz, #sc-166075), PVEGFR2 (CST, #2478), P-VEGFR3 (Cell application, # CY1115) P-ERK1/2 (CST #4370), P-JNK (CST #4668), P-eNOS (CST #9571), P-Smad 1/5 (CST, #9516), P-AKT (CST #9271), NRP1 (R&D Systems, #AF566), VEGFR2 (R&D Systems, #AF357), ADAMTS1 (R&D Systems, #AF5867), VEGFR3 (R&D Systems, #AF349), NICD (CST #4147), NTM (CST #3608), P-Smad2/3 (Sigma #04-953), -actinin (CST #6487), -tubulin (Sigma #T9026). .. Then, primary antibodies were revealed using horseradish peroxidase-coupled anti-mouse, anti-goat, anti-sheep or anti-rabbit (Jackson ImmunoResearch) secondary antibodies for 1 h. Finally, horseradish peroxidase activity was revealed using a chemiluminescence kit (GE-healthcare) according to the manufacturer’s instructions.

    Concentration Assay:

    Article Title: VEGF-C-mediated Cardiac Lymphangiogenesis Promotes Inflammation Resolution in Autoimmune Acute Myocarditis in Mice
    Article Snippet: .. Sections were then incubated with the following primary antibodies: D2-40 (anti-Podoplanin) (413151, Nichirei Biosciences, at its original concentration (ready to use product)), VEGFR3 (R&D Systems, AF743, 1:150, RRID: AB_355563), CD68 (Cell Signaling Technology, 97778, 1:150, RRID: AB_2928056), CD3 (Abcam, ab5690, 1:150, RRID: AB_305055), CD4 (Abcam, ab183685, 1:150, RRID: AB_2686917), CD8 (Abcam, ab209775, 1:150, RRID: AB_2860566), Foxp3 (Abcam, ab215206, 1:100, RRID: AB_2860568), CD20 (Abcam, ab64088, 1:300, RRID: AB_1139386), CD11c (Cell Signaling Technology, 97585, 1:100, RRID: AB_2800282), iNOS (Cell Signaling Technology, 68186, 1:500, RRID: AB_3662912), Arginase1 (Cell Signaling Technology, 93668, 1:100, RRID: AB_2800207), CCR2 (Abcam, ab273050, 1:150, RRID:A AB_2893307), Galectin-3 (Cell Signaling Technology, 89572, 1:100, RRID: AB_2800111), SPP1 (Cell Signaling Technology, 88742, 1:100, RRID: AB_3107209), MRC1 (Abcam, ab64693, 1:100, RRID:AB_1523912), and Ki67 (Abcam, ab15580, 1:250, RRID: AB_443209). ..

    Lysis:

    Article Title: Supporting Information for Fibrillin-1 regulates endothelial sprouting during angiogenesis
    Article Snippet: .. Lysis was performed directly in Laemmli sample buffer and samples were run on a 10% SDS-PAGE gel at 120 V for 90 min. Proteins were transferred onto Immobilon polyvinylidene difluoride membranes (Merck Millipore) at 120 V for 1 h. Membranes were saturated with 5% bovine serum albumin in Tris-buffered saline containing 0.1 % Tween 20 for 1h and incubated overnight at 4°C with primary antibodies against fibronectin (Abcam, #ab6328), Dll4 (Cell Signaling Technologies (CST) #2589), Jagged-1 (CST #70109), Smad1 (CST #9743), Smad6 (Novus, #NB100-56440), MAGP1 (Santa Cruz, #sc-166075), PVEGFR2 (CST, #2478), P-VEGFR3 (Cell application, # CY1115) P-ERK1/2 (CST #4370), P-JNK (CST #4668), P-eNOS (CST #9571), P-Smad 1/5 (CST, #9516), P-AKT (CST #9271), NRP1 (R&D Systems, #AF566), VEGFR2 (R&D Systems, #AF357), ADAMTS1 (R&D Systems, #AF5867), VEGFR3 (R&D Systems, #AF349), NICD (CST #4147), NTM (CST #3608), P-Smad2/3 (Sigma #04-953), -actinin (CST #6487), -tubulin (Sigma #T9026). .. Then, primary antibodies were revealed using horseradish peroxidase-coupled anti-mouse, anti-goat, anti-sheep or anti-rabbit (Jackson ImmunoResearch) secondary antibodies for 1 h. Finally, horseradish peroxidase activity was revealed using a chemiluminescence kit (GE-healthcare) according to the manufacturer’s instructions.

    SDS Page:

    Article Title: Supporting Information for Fibrillin-1 regulates endothelial sprouting during angiogenesis
    Article Snippet: .. Lysis was performed directly in Laemmli sample buffer and samples were run on a 10% SDS-PAGE gel at 120 V for 90 min. Proteins were transferred onto Immobilon polyvinylidene difluoride membranes (Merck Millipore) at 120 V for 1 h. Membranes were saturated with 5% bovine serum albumin in Tris-buffered saline containing 0.1 % Tween 20 for 1h and incubated overnight at 4°C with primary antibodies against fibronectin (Abcam, #ab6328), Dll4 (Cell Signaling Technologies (CST) #2589), Jagged-1 (CST #70109), Smad1 (CST #9743), Smad6 (Novus, #NB100-56440), MAGP1 (Santa Cruz, #sc-166075), PVEGFR2 (CST, #2478), P-VEGFR3 (Cell application, # CY1115) P-ERK1/2 (CST #4370), P-JNK (CST #4668), P-eNOS (CST #9571), P-Smad 1/5 (CST, #9516), P-AKT (CST #9271), NRP1 (R&D Systems, #AF566), VEGFR2 (R&D Systems, #AF357), ADAMTS1 (R&D Systems, #AF5867), VEGFR3 (R&D Systems, #AF349), NICD (CST #4147), NTM (CST #3608), P-Smad2/3 (Sigma #04-953), -actinin (CST #6487), -tubulin (Sigma #T9026). .. Then, primary antibodies were revealed using horseradish peroxidase-coupled anti-mouse, anti-goat, anti-sheep or anti-rabbit (Jackson ImmunoResearch) secondary antibodies for 1 h. Finally, horseradish peroxidase activity was revealed using a chemiluminescence kit (GE-healthcare) according to the manufacturer’s instructions.

    Saline:

    Article Title: Supporting Information for Fibrillin-1 regulates endothelial sprouting during angiogenesis
    Article Snippet: .. Lysis was performed directly in Laemmli sample buffer and samples were run on a 10% SDS-PAGE gel at 120 V for 90 min. Proteins were transferred onto Immobilon polyvinylidene difluoride membranes (Merck Millipore) at 120 V for 1 h. Membranes were saturated with 5% bovine serum albumin in Tris-buffered saline containing 0.1 % Tween 20 for 1h and incubated overnight at 4°C with primary antibodies against fibronectin (Abcam, #ab6328), Dll4 (Cell Signaling Technologies (CST) #2589), Jagged-1 (CST #70109), Smad1 (CST #9743), Smad6 (Novus, #NB100-56440), MAGP1 (Santa Cruz, #sc-166075), PVEGFR2 (CST, #2478), P-VEGFR3 (Cell application, # CY1115) P-ERK1/2 (CST #4370), P-JNK (CST #4668), P-eNOS (CST #9571), P-Smad 1/5 (CST, #9516), P-AKT (CST #9271), NRP1 (R&D Systems, #AF566), VEGFR2 (R&D Systems, #AF357), ADAMTS1 (R&D Systems, #AF5867), VEGFR3 (R&D Systems, #AF349), NICD (CST #4147), NTM (CST #3608), P-Smad2/3 (Sigma #04-953), -actinin (CST #6487), -tubulin (Sigma #T9026). .. Then, primary antibodies were revealed using horseradish peroxidase-coupled anti-mouse, anti-goat, anti-sheep or anti-rabbit (Jackson ImmunoResearch) secondary antibodies for 1 h. Finally, horseradish peroxidase activity was revealed using a chemiluminescence kit (GE-healthcare) according to the manufacturer’s instructions.



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    Image Search Results


    Neutralizing VEGF-A or Blocking VEGFR2 Abrogates the Protective Effect of Catalpol-Pretreated MMCs in vitro. ( A , B) Immunohistochemical staining images showing the expression of VEGFR2 in the kidneys of mice from different groups (400×). ( C , D ) Immunofluorescence staining images showing the expression of VEGFR2 in TCMK-1 cells from different groups (400×). ( E , F ) CCK-8 and LDH assay results showing the proliferative activity and cytotoxicity of TCMK-1 cells in different groups ( n = 6). ( G , H ) Western blot showing the expression of the inflammatory markers p-p65 and TNF-α in cells from different groups and statistical analysis ( n = 3). ( I - P ) Levels of the oxidative stress indicators ROS and T-SOD and ROS staining in TCMK-1 cells ( n = 3). ( Q - R ) Western blot showing the expression of the necroptosis markers RIP, RIP3, and MLKL, as well as their phosphorylated forms, in cells from different groups and statistical analysis ( n = 3). CON : control group; CP : cisplatin injury group; cata-CM : catalpol-pretreated MMC supernatant group; VEGF-ab : catalpol-pretreated MMC supernatant + VEGF-A neutralizing antibody group; Ki8751: catalpol-pretreated MMC supernatant + VEGFR2 inhibitor Ki8751 group; MAZ51: catalpol-pretreated MMC supernatant + VEGFR3 inhibitor MAZ51 group. *** P < 0.001, ** P < 0.01, * P < 0.05

    Journal: Stem Cell Research & Therapy

    Article Title: Pretreatment of metanephric mesenchymal cells with catalpol mitigates acute kidney injury through VEGF-A secretion via multiple mechanisms

    doi: 10.1186/s13287-026-04914-9

    Figure Lengend Snippet: Neutralizing VEGF-A or Blocking VEGFR2 Abrogates the Protective Effect of Catalpol-Pretreated MMCs in vitro. ( A , B) Immunohistochemical staining images showing the expression of VEGFR2 in the kidneys of mice from different groups (400×). ( C , D ) Immunofluorescence staining images showing the expression of VEGFR2 in TCMK-1 cells from different groups (400×). ( E , F ) CCK-8 and LDH assay results showing the proliferative activity and cytotoxicity of TCMK-1 cells in different groups ( n = 6). ( G , H ) Western blot showing the expression of the inflammatory markers p-p65 and TNF-α in cells from different groups and statistical analysis ( n = 3). ( I - P ) Levels of the oxidative stress indicators ROS and T-SOD and ROS staining in TCMK-1 cells ( n = 3). ( Q - R ) Western blot showing the expression of the necroptosis markers RIP, RIP3, and MLKL, as well as their phosphorylated forms, in cells from different groups and statistical analysis ( n = 3). CON : control group; CP : cisplatin injury group; cata-CM : catalpol-pretreated MMC supernatant group; VEGF-ab : catalpol-pretreated MMC supernatant + VEGF-A neutralizing antibody group; Ki8751: catalpol-pretreated MMC supernatant + VEGFR2 inhibitor Ki8751 group; MAZ51: catalpol-pretreated MMC supernatant + VEGFR3 inhibitor MAZ51 group. *** P < 0.001, ** P < 0.01, * P < 0.05

    Article Snippet: Ultimately, 5 ng/ml VEGF-A (HY- P72280 , MCE, USA), 0.1 μg/ml VEGF-A neutralizing antibody (sc-7249, Santa Cruz, USA), 10 nM VEGFR2 blocker Ki8751 (HY-12038, MCE, USA), and 5 μM VEGFR3 blocker MAZ51 (HY-116624, MCE, USA) were used.

    Techniques: Blocking Assay, In Vitro, Immunohistochemical staining, Staining, Expressing, Immunofluorescence, CCK-8 Assay, Lactate Dehydrogenase Assay, Activity Assay, Western Blot, Control

    Pretreatment of MMCs with catalpol may suppress the STAT3 pathway and activate the p38 pathway. ( A , B ) Western blot showing the expression of STAT3, p38, and their phosphorylated proteins in kidney tissues from mice in different groups ( n = 6). Ctrl: control group; CP: cisplatin model group; MMC-cata: catalpol-pretreated MMC treatment group; siRNA3: siRNA3-transfected MMC treatment group. ( C , D ) Western blot showing the expression of STAT3, p38, and their phosphorylated proteins in cells from different groups ( n = 3). CON: control group; CP: cisplatin injury group; cata-CM: catalpol-pretreated MMC supernatant group; VEGF-ab: catalpol-pretreated MMC supernatant + VEGF-A neutralizing antibody group; Ki8751: catalpol-pretreated MMC supernatant + VEGFR2 inhibitor Ki8751 group; MAZ51: catalpol-pretreated MMC supernatant + VEGFR3 inhibitor MAZ51 group. ( E – F ) Western blot showing the expression of STAT3, p38, and their phosphorylated proteins in cells from different groups ( n = 3). CON: control group; CP: cisplatin injury group; VEGF-A: VEGF-A treatment group; and Ki8751: VEGF-A + VEGFR2 inhibitor Ki8751 group. *** P < 0.001, ** P < 0.01, * P < 0.05

    Journal: Stem Cell Research & Therapy

    Article Title: Pretreatment of metanephric mesenchymal cells with catalpol mitigates acute kidney injury through VEGF-A secretion via multiple mechanisms

    doi: 10.1186/s13287-026-04914-9

    Figure Lengend Snippet: Pretreatment of MMCs with catalpol may suppress the STAT3 pathway and activate the p38 pathway. ( A , B ) Western blot showing the expression of STAT3, p38, and their phosphorylated proteins in kidney tissues from mice in different groups ( n = 6). Ctrl: control group; CP: cisplatin model group; MMC-cata: catalpol-pretreated MMC treatment group; siRNA3: siRNA3-transfected MMC treatment group. ( C , D ) Western blot showing the expression of STAT3, p38, and their phosphorylated proteins in cells from different groups ( n = 3). CON: control group; CP: cisplatin injury group; cata-CM: catalpol-pretreated MMC supernatant group; VEGF-ab: catalpol-pretreated MMC supernatant + VEGF-A neutralizing antibody group; Ki8751: catalpol-pretreated MMC supernatant + VEGFR2 inhibitor Ki8751 group; MAZ51: catalpol-pretreated MMC supernatant + VEGFR3 inhibitor MAZ51 group. ( E – F ) Western blot showing the expression of STAT3, p38, and their phosphorylated proteins in cells from different groups ( n = 3). CON: control group; CP: cisplatin injury group; VEGF-A: VEGF-A treatment group; and Ki8751: VEGF-A + VEGFR2 inhibitor Ki8751 group. *** P < 0.001, ** P < 0.01, * P < 0.05

    Article Snippet: Ultimately, 5 ng/ml VEGF-A (HY- P72280 , MCE, USA), 0.1 μg/ml VEGF-A neutralizing antibody (sc-7249, Santa Cruz, USA), 10 nM VEGFR2 blocker Ki8751 (HY-12038, MCE, USA), and 5 μM VEGFR3 blocker MAZ51 (HY-116624, MCE, USA) were used.

    Techniques: Western Blot, Expressing, Control, Transfection

    Effects of magnesium extracts on lymphangiogenesis-related gene and protein expression in LECs. ( A ) Relative Vegfa mRNA expression levels in LECs treated with Control, Mg (1:10), Mg (1:100), and Mg (1:1000), as determined by qRT-PCR. ( B ) Relative Vegfc mRNA expression levels in LECs under the same treatment conditions. ( C ) Representative Western blot images showing VEGFA and VEGFC protein expression in LECs after treatment with different concentrations of magnesium extracts, with GAPDH used as the internal loading control. ( D ) Additional Western blot analysis showing VEGFR3 protein expression under the same treatment conditions, with β-actin used as the internal loading control. Data in ( A , B ) are presented as the mean ± SD ( n = 3). Statistical analysis for ( A , B ) was performed using one-way ANOVA followed by Tukey’s multiple-comparisons test. Statistical significance is indicated as * p < 0.05, ** p < 0.01; ns, not significant.

    Journal: Biomedicines

    Article Title: Ionic Extracts of Magnesium Powders Promote In Vitro Lymphangiogenesis

    doi: 10.3390/biomedicines14040913

    Figure Lengend Snippet: Effects of magnesium extracts on lymphangiogenesis-related gene and protein expression in LECs. ( A ) Relative Vegfa mRNA expression levels in LECs treated with Control, Mg (1:10), Mg (1:100), and Mg (1:1000), as determined by qRT-PCR. ( B ) Relative Vegfc mRNA expression levels in LECs under the same treatment conditions. ( C ) Representative Western blot images showing VEGFA and VEGFC protein expression in LECs after treatment with different concentrations of magnesium extracts, with GAPDH used as the internal loading control. ( D ) Additional Western blot analysis showing VEGFR3 protein expression under the same treatment conditions, with β-actin used as the internal loading control. Data in ( A , B ) are presented as the mean ± SD ( n = 3). Statistical analysis for ( A , B ) was performed using one-way ANOVA followed by Tukey’s multiple-comparisons test. Statistical significance is indicated as * p < 0.05, ** p < 0.01; ns, not significant.

    Article Snippet: The primary antibodies used in this study included anti-VEGFA rabbit polyclonal antibody (ZEN-BIOSCIENCE Co., Ltd., Chengdu, China), anti-VEGFC rabbit polyclonal antibody (ZEN-BIOSCIENCE Co., Ltd., Chengdu, China), anti-VEGFR3 rabbit polyclonal antibody (Affinity Biosciences, Shanghai, China), anti-GAPDH rabbit polyclonal antibody (Affinity Biosciences, Shanghai, China), and anti-β-actin rabbit polyclonal antibody (Affinity Biosciences, Shanghai, China).

    Techniques: Expressing, Control, Quantitative RT-PCR, Western Blot